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    您現在的位置:首頁 > 產品中心 > 細胞庫 > 兔腎細胞系 > CCL-37 RK-13 兔腎細胞系

    CCL-37 RK-13 兔腎細胞系

    • 更新時間:  2020-10-15
    • 產品型號:  
    • 簡單描述
    • CCL-37 RK-13 兔腎細胞系,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和Z優培養條件
    詳細介紹

    CCL-37 RK-13 兔腎胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背細胞系,ATCC 細景清楚,提供參考文獻和優培養條件

     

    ATCC® Number:CCL-37™   Price:$339.00
    Designations:RK13
    Depositors: BC Meyer
    Biosafety Level:2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]
    Shipped:frozen
    Medium & Serum:See Propagation
    Growth Properties:adherent
    Organism:Oryctolagus cuniculus (rabbit)
    Morphology:epithelial
     
    Source:Organ: kidney
    Disease: normal
    Cellular Products:keratin
    Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
     
    Applications:transfection host (Roche FuGENE® Transfection Reagents)
    Age:5 weeks        CCL-37 RK-13 兔腎細胞系
    Comments:The cells are positive for keratin by immunoperoxidase staining.The cells are positive for bovine viral diarrhea virus (BVDV).
    Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Subculturing:Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37C.

    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
    Medium Renewal: Twice per week
    Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
    recommended serum:ATCC 30-2020
    0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
    Cell culture tested DMSO:ATCC 4-X
    References:18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708
    22145: . . Lancet 2: 640, 1963. 
    22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
    26192: . . Lancet 2: 593, 1963.
    28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321        

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